Not available outside of the UK & Ireland.

Application

Immunization

General description

Contains 10 vials per set, each containing 10 ml of Freund′s complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.

1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Compositionr>The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:r>85% Drakeol 5 NFr>15% Arlacel A, mannide monooleate emulsifierr>0.1% Mycobacterium butyricum dry cellsr>r>Sample Preparationr>1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.r>2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.r>3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.r>4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.

Packaging

1 set in Glass bottle

Physical form

Yellow, oily, bacterial suspension.

Warning

Toxicity: Standard Handling (A)