Not available outside of the UK & Ireland.

Biochem/physiol Actions

1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.

General description

Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.

M.W. α-subunit: ~48,000; β-subunit: ~46,000

Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras. Recognizes the C-terminal CAAX cysteine motif where the farnesylated cysteine is followed by two aliphatic amino acids and either leucine or serine.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hightower, K.E., et al. 2001. Biochem. J.360, 625.Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.Reiss, Y., et al. 1990. Cell62, 81.

Packaging

100 µg in Plastic ampoule

Physical form

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)