Not available outside of the UK & Ireland.

Analysis Note

single band by SDS-PAGE

General description

Native fibrinogen fragment D from human plasma prepared by plasmin digestion of purified fibrinogen followed by preparative electrophoresis. Thermolabile core fragment of fibrinogen. Complete proteolysis of a single fibrinogen molecule produces two D and one E fragments. The D and E fragments have no common antigenic determinants; therefore, no cross-reaction occurs. Reported to increase endothelial membrane permeability to albumin.

Native fibrinogen fragment D from human plasma prepared by plasmin digestion of purified fibrinogen followed by preparative electrophoresis. Thermolabile fragment. Complete proteolysis of a single fibrinogen molecule produces two D and one E fragments. Fragment D has been shown to increase endothelial monolayer permeability and to decrease polymorphonuclear leukocyte adhesion to endothelial cells. The D and E fragments have no common antigenic determinants; therefore, no cross-reactivity occurs.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Spraggon, G., et al. 1997. Nature 389, 455.Stirk, C.M., et al. 1993. Atherosclerosis103, 159.Vogel, P., et al. 1993. Eur. Surg. Res.25, 83.Azpiazu, I., and Chapman, D. 1992. Biochim. Biophys. Acta1119, 268.Thompson, W.D., et al. 1992. J. Pathol.168, 47.Fisher, E.G., et al. 1991. Haemostasis21, 141.Ge, M., et al. 1991. Am. J. Physiol.261, L283.

Packaging

200 µg in Plastic ampoule

Physical form

Lyophilized from 400 mM glycine, 150 mM NaCl.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 4 h at 20°C or for up to 8 h at 4°C or for up to 3 months at -70°C.

Warning

Toxicity: Standard Handling (A)