Not available outside of the UK & Ireland.

Application

ELISA (1:1000)r>Immunofluorescence (1:50)r>Immunoblotting (not recommended; see comments)

General description

Purified rabbit polyclonal antibody. Recognizes farnesyl.

Anti-Farnesyl, rabbit polyclonal, recognizes farnesyl in a wide variety of species. Also recognizes KLH. It is validated for use in ELISA and immunofluorescence.

Recognizes farnesyl. Also recognizes KLH.

Immunogen

farnesyl cysteine conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Baron, R., et al. 2000. Proc. Natl. Acad. Sci. USA97, 11626.Lin, H.P., et al. 1999. J. Gen. Virol.80, 91.Aspbury, R.A., et al. 1998. Biochim Biophys Acta1392, 265.Gromov, P.S., et al. 1996. Electrophoresis17, 1728.Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem.65, 241.

Recognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)