Not available outside of the UK & Ireland.

General description

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M^-1cm^-1.

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.Yamada, H., et al. 1981. Biochemistry20, 4836.Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.Ozawa, H. 1970. Biochemistry9, 2158.Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

Packaging

Packaged under inert gas

25 g in Glass bottle

5 g in Plastic ampoule

Preparation Note

Avoid amine containing buffers.

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Warning

Toxicity: Irritant (B)