Not available outside of the UK & Ireland.

General description

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.Crostella, L., et al. 2001. Oncogene20, 3735.Guiton, M., et al. 1994. J. Biol. Chem269, 30370.

Packaging

10 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).

Warning

Toxicity: Standard Handling (A)