Not available outside of the UK & Ireland.

Application

Immunoblotting (1 µg/ml)r>Immunoprecipitation (see comments)

General description

This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

Recognizes the ~97 kDa glycosylated α-ENaC protein.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.

Immunogen

a synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human A-epithelial cell sodium channel (A-ENaC), conjugated to carrier protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London520, 631.Mano, I., and Driscoll, M. 1999. Bioessays21, 568.Fyfe, G.K., et al. 1998. Semin. Nephrol.18, 138.

Does not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)