Not available outside of the UK & Ireland.

Analysis Note

Positive ControlA431 cells stimulated with EGF

Application

Immunoblotting (1:1000)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr⁸⁴⁵.

Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr⁸⁴⁵ in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.

Packaging

10 tests in Plastic ampoule

Physical form

In Dulbecco′s PBS (w/o Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)