Not available outside of the UK & Ireland.

General description

Recombinant, Streptococcus pneumoniae Endo-A-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.

Note: 1 mU = 1 milliunit.

Recombinant, Streptococcus pneumoniae Endo-A-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.

Packaging

50 miu in Plastic ampoule

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-A-D-galactopyranoside per min at 37°C, pH 5.0.

Warning

Toxicity: Standard Handling (A)