Not available outside of the UK & Ireland.

Analysis Note

Positive ControlRat brain

Application

ELISA (see comments)r>Flow Cytometry (1:100, see application references)r>Immunoblotting (1:200)r>Immunohistochemistry (1:2500)

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D₅ dopamine receptor protein.

Anti-Dopamine D5 Receptor, rabbit polyclonal, recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.

Recognizes the dopamine D₅ receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

a synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Warning

Toxicity: Standard Handling (A)