Not available outside of the UK & Ireland.

Analysis Note

Positive ControlRat brain

Application

ELISA (see comments)r>Immunoblotting (1:500)r>Immunohistochemistry (1:10,000)

General description

Recognizes the ~48-51 kDa dopamine D₄ receptor in rat brain. Does not cross-react with other dopamine receptors.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D₄ dopamine receptor.

Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.

Immunogen

a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.

Warning

Toxicity: Standard Handling (A)