Not available outside of the UK & Ireland.

Analysis Note

Comparable to reference lot by SDS-PAGE

General description

Native calpain-1 from human erythrocytes. Ca²⁺-dependent heterodimeric cysteine proteinase with low Ca²⁺ requirement (EC₅₀= 2 µM).

Native calpain-1 from human erythrocytes. Ca²⁺-dependent cysteine proteinase with low Ca²⁺ requirement (half-maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol.81, 323.Sorimachi, H., et al. 1997. Biochem. J. 328, 721.Croall, D.E., and McGrody, K.S. 1994. Biochemistry33, 13223.

Packaging

Please refer to vial label for lot-specific concentration.

500 µg in Plastic ampoule

Physical form

In 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Preparation Note

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit.

Warning

Toxicity: Harmful (C)