Not available outside of the UK & Ireland.

Application

Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using: Dot blotsELISAImmunocytochemistryImmunoprecipitationWestern blotsSince Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

General description

Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.The following concentrations should be taken as a guideline: ELISA: for detection 100 ng/ml; for coating 1 to 5 µg/ml Immunoprecipitation: 0.5 to 5 µg/ml Western blot: 50 to 200 ng/ml

Quality

Function tested in western blot.

Reconstitution

Add 0.5 ml (11 867 423 001; 50 µg) or 2.5 ml (11 867 431 001; 500 µg) double-distilled water to a final concentration of 100 µg/ml or 200 µg/ml respectively.Rehydrate for 10 min prior to use.

Specificity

Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).