Not available outside of the UK & Ireland.

Application

Anti-phospho-Akt (Ser473) Antibody, clone 6F5 detects level of phospho-Akt (Ser473) & has been published & validated for use in ELISA, WB, FC, IF, IH.

Immunofluroescence Analysis:
EGF-treated A431 cells were fixed, permeabilized, and stained with Anti-phospho-Akt (Ser473), clone 6F5 (2 µg/mL) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor™488 (green) was used to stain actin.

Immunohistochemistry:
Anti-phospho-Akt (Ser473), clone 6F5 was diluted to 1:200, IHC-Select Detection with HRP-DAB.

Flow Cytometry:
Anti-phospho-Akt (Ser473) was used at a 1:800 dilution for this assay.

Immunogen

Peptide encompassing and including phosphorylated Ser473 of human Akt1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05%NaN3

Quality

Western Blot Analysis:
0.5-1 µg/mL of this lot detected phosphorylated Akt in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.

Specificity

Expected to react with most species as sequence is 100% homologous with most common vertebrate.

Recognizes Akt only when phosphorylated on Ser473. This antibody does not cross-react with Akt1/PKBα when it is not phosphorylated on Ser473.

Target description

60 kDa