G 418 disulfate salt solution, 50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture

Code: g8168-100ml D2-231

Not available outside of the UK & Ireland.

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal ...


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£678.00 100ML
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Not available outside of the UK & Ireland.

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Caution

The product is stable for two years when stored at 2-8°C.

General description

Chemical structure: aminoglycoside

antibiotic activity spectrumparasites
biological sourceMicromonospora echinospora
colorcolorless
concentration50 mg/mL in H2O
formsolution
impuritiesendotoxin, tested
InChI keyUHEPSJJJMTWUCP-NKCAIAFTSA-N
InChI1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
mode of actionprotein synthesis | interferes
mol wt692.7
packagingpkg of 10 and 100 mL
potency50-60 mg per mL
product lineBioReagent
Quality Level200
SMILES stringOS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
solubilityH2O: 50 mg/mL, clear
sterility0.1 µm filtered
storage temp.2-8°C
technique(s)cell culture | mammalian: suitable
Code
Description
Unit Size
List Price
Qty
G8168-10ML
G 418 disulfate salt solution, 50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture

Unit:10ML
List Price: £129.00
Source:List Price
ADD
G8168-50ML
G 418 DISULFATE SALT SOLUTION

Unit:50ML
List Price: £442.00
Source:List Price
ADD
Cas Number108321-42-2
This product has met the following criteria: