Chloramphenicol, >=98% (TLC)

Code: c0378-500g D2-231

Not available outside of the UK & Ireland.

Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biolog...


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$619.15 500G

Not available outside of the UK & Ireland.

Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes. Chloramphenicol has been used as a bacteriostatic non potent antibiotic. It has been used for the selection/growth of positive bacterial cells.

Biochem/physiol Actions

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription. Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it. Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Caution

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

General description

Chemical structure: phenicole

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Packaging

1 kg in poly bottle

5, 25, 100, 500 g in poly bottle

Preparation Note

Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

antibiotic activity spectrummycoplasma, Gram-positive bacteria, Gram-negative bacteria, mycobacteria
assay≥98% (HPLC)
formpowder or crystals
Gene Informationhuman ... CYP1A2(1544)
InChI keyWIIZWVCIJKGZOK-RKDXNWHRSA-N
InChI1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
mode of actionprotein synthesis | interferes
mp148-150 °C (lit.)
pKa 5.5
Quality Level300
SMILES stringOC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O
storage temp.room temp
Code
Description
Unit Size
List Price
Qty
C0378-25G
Unit:25G
List Price: $55.39
Source:List Price
ADD
C0378-5G
Unit:5G
List Price: $28.42
Source:List Price
ADD
C0378-100G
Unit:100G
List Price: $169.65
Source:List Price
ADD
C0378-1KG
Unit:1KG
List Price: $958.45
Source:List Price
ADD
Cas Number56-75-7
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