HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

• Optimized for convenient scale-up purification of histidine-tagged proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible leakage of Ni²⁺.
• High binding capacity, approx. 40mg/mL resin.
• Convenient and time-saving 20mL prepacked HiPrep format.

HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.