StrepTactin™ Sepharose High Performance resin is designed for Strep-tag II™ recombinant protein purification.
- Yields high-purity tagged recombinant proteins eluted in concentrated, small volumes
- Physiological conditions and mild elution preserve target protein activity
- Fast and easy regeneration with 0.5M NaOH
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Stable in all commonly used buffers
StrepTactin™ Sepharose agarose resin
Strep-tag II™ is small (8 aa), which often makes tag removal unnecessary after purification. StrepTactin™ Sepharose High Performance has been specially developed for high specificity towards Strep-tag II™ to ensure purity.
The resin’s small bead size (34µm) allows elution in narrow peaks. Purification and elution conditions are designed to be mild and mimic physiological conditions, preserving the target protein’s activity.
This robust and stable Sepharose-based resin yields reproducible purifications and minimizes the need for further concentration steps. See our StrepTactin™ Sepharose application note for an example of a single-step purification that achieved greater than 95% purity.
This product has met the following criteria: