Protein A/G HP SpinTrap Buffer Kit

Code: 28913567 D2-84

Pre-made buffers optimized for the affinity-based enrichment of a target protein/immunoprecipitation using Protein A HP, Protein G HP, and Ab SpinTrap columns.

REQUEST

Pre-made buffers optimized for the affinity-based enrichment of a target protein/immunoprecipitation using Protein A HP, Protein G HP, and Ab SpinTrap columns.

  • High quality buffers.
  • All buffer components listed and defined.
  • Convenient format - stock solutions can be diluted directly into the provided bottles for storage.
  • Eliminates time-consuming buffer preparation, which allows fast, reproducible protein enrichment.

The buffers provided in the kit are optimized for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with Protein A HP SpinTrap, Protein G HP SpinTrap, and Ab SpinTrap columns

Protein A/G HP SpinTrap Buffer Kit
Storage conditions4 - 30°C
Certificate of AnalysisYes
Wetted PartNo
CapacitySufficient for performing 16 reactions
Kit contents2 x 5mL Binding/washing buffer (TBS) (containing 0.5M Tris and 1.5M NaCl,pH 7.5) 1 x 20mL Elution buffer (classic)(containing 2.5% HAc) 1 x 3 ml Elution buffer (crosslink) (containing 1 M Glycine-HCl, pH 2.9) 1 x 4mL Crosslink solution A (containing 2M Triethanolamine, pH 8.9) 2 x 1mL Crosslink solution B (containing 1M Ethanolamine pH 8.9) Instructions
LigandProtein A
ScaleLab
ApplicationOther
FormatBuffer
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