Heparin Sepharose 6 Fast Flow 50 ml

Code: 17099801 D2-84

Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.

  • Proven for industrial pla...


    read more

    Your Price
    £719.00 EACH
    £862.80 inc. VAT

Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.

  • Proven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors.
  • Based on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
  • Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Applications of heparin affinity chromatography

Cytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.

Chemically stable heparin purification resin

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.

Heparin Sepharose 6 Fast Flow
Certificate of AnalysisYes
BioProcess resinYes
LigandPorcine heparin
Particle size, d50V~90µm
Matrixcross-linked agarose, 6%, spherical
Ligand Concentration~4mg heparin/mL resin
Ligand Coupling MethodReductive Amination
pH stability, operational4 - 12
pH stability, CIP4 - 13
Pressure/flow characteristics250-400cm/h at 0.1MPa in a XK 50/60 column with 5cm diameter and 25cm bed height (at 20°C using buffers with the same viscosity as water)
Storage4 to 30°C, 20% Ethanol, 0.05M Sodium Acetate
Chemical stabilityStable in commonly used aqueous buffers - 0.1M NaOH, 0.05M sodium acetate, 8M urea, 6M guanidine hydrochloride, non-ionic detergents, 10% glycerol
Autoclavability30min at 121°C in 0.02M NaH2PO4, pH 7, 5 cycles
Chromatography techniqueGroup specific affinity
This product has met the following criteria: