GammaBind Plus Sepharose 25 ml

Code: 17088602 L1-23

GammaBind Plus Sepharose is a protein G affinity chromatography resin for purifying immunoglobulins (IgGs) from a variety of species.

  • Enhanced binding to rat and mouse IgG subclas...


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    £3,887.00 EACH

GammaBind Plus Sepharose is a protein G affinity chromatography resin for purifying immunoglobulins (IgGs) from a variety of species.

  • Enhanced binding to rat and mouse IgG subclasses compared with GammaBind G Sepharose.
  • Binds to all mouse, rat, and human IgG subclasses; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  • Lab-scale IgG purification yields up to 35mg human IgG/mL.
  • Well suited for immunoprecipitation procedures.

Affinity chromatography resin for IgG purification

GammaBind Plus Sepharose is used to analyze and purify classes, subclasses, and fragments of immunoglobulins from any biological fluid or cell culture medium. This resin is also suitable for immunoprecipitation.

This affinity chromatography resin is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by maleimide linkage. This recombinant form of streptococcal protein G binds strongly to the Fc region of IgG from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on Protein G.

GammaBind Plus Sepharose
Flow velocity, cm/h<130cm/h
LigandRecombinant GammaBind G type 3 lacking albumin-binding region
Particle size, d50V~90µm
MatrixCross-linked agarose, 6%
Ligand Coupling MethodMaleimide activation
Ligand density3mg GammaBind G, type 3/mL medium
pH stability, operational3 - 9
pH stability, CIP2 - 9
Storage2 to 8°C, 20% Ethanol
Chemical stabilityCommonly used aqueous buffers
Chromatography techniqueAntibody affinity chromatography
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17088601
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17088604
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