Lentil Lectin Sepharose 4B 25 ml

Code: 17044401 D2-84

Lentil Lectin Sepharose 4B resin is used for glycoprotein purification and purification of other carbohydrate-containing molecules.

  • Binds molecules that contain α-D-mannose, ...


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Lentil Lectin Sepharose 4B resin is used for glycoprotein purification and purification of other carbohydrate-containing molecules.

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related sugars.
  • Compared to Con A, lentil lectin distinguishes less sharply between glucosyl and mannosyl residues.
  • Retains binding in 1% deoxycholate and is valuable for purifying detergent-solubilized membrane proteins.

Capture of proteins with affinity for lentil lectin

Lentil lectin is a metalloprotein that binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a group-specific adsorbent that is well-suited to purifying membrane proteins and viral glycoproteins.

Lentil Lectin Sepharose 4B 25 mL
Particle Size, µm45 - 165
Flow velocity 75cm/h at 25°C, HR 16/10 column, 5cm bed height
LigandLentil lectin
Particle size, d50V~90µm
MatrixAgarose, 4%
Ligand Coupling MethodCyanogen bromide activation
Ligand density2mg Lentil lectin/mL medium
ApplicationFor purification, isolation or removal of molecules containing branched mannoses with fucose linked α(1,6) to the N-acetylglucosamine, (αMan>αGlc>GlcNAc) and sterically related residues like glycoproteins, membrane proteins, glycolipids, lipoproteins, pol
pH stability, operational3 - 10
pH stability, CIP3 - 10
Pressure/Flow SpecificationBase matrix 70-140cm/h, pressure drop cm H2O/bed height=15, bed height 10cm, 5cm i.d.
Storage2 to 8°C, 20% Ethanol, 0.9% NaCl, 1mM Ca2+ and 1mM Mn2+
Chemical stabilityStable to commonly used aqueous buffers. Chelating agents such as EDTA, 8M urea or solutions having a pH below 3 should be avoided as these conditions result in removal of manganese from the lectin and loss of activity
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