Epoxy-Activated Sepharose 6B 15 g

Code: 17048001 D2-84

Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 1...


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Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm

  • Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups

Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands. Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Other ligands can be coupled through hydroxyl, amino or thiol groups. The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. Epoxy-activated Sepharose 6B is formed by reacting Sepharose 6B with 1,4-bis (2,3- epoxy-propoxy-) butane.

Epoxy-Activated Sepharose 6B
Particle Size, µm45 - 165
Flow Velocity75cm/h at 25°C, HR 16/10 column, 5cm bed height
Particle Size, d50V~90µm
Group to be Coupled-NH2, -OH, or -SH
MatrixAgarose, 6%
ApplicationThe resin has a long hydrophilic spacer arm which makes it particularly suitable for immobilization of small molecules
Pressure/Flow SpecificationBase Matrix 100-200cm/h, pressure drop cm H2O/bed height=15, bed height 10cm, 5cm i.d.
Storage2 to 8°C
Chemical StabilityStable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8M urea and 6M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane
Chromatography TechniqueAffinity coupling
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