Guanidine hydrochloride, organic base and chaeotropic agent, >=99% (titration)

Code: G4505-500G D2-231

Not available outside of the UK & Ireland.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins su...


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Your Price
$253.75 500G

Not available outside of the UK & Ireland.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Guanidine hydrochloride (GdnHCl) has been used: to perform absolute quantitation of amyloid-beta (Aß) isoforms in plasmain protein carbonylation assayfor the quantification of glycosaminoglycans (GAG)for denaturation of cow’;s milk allergensas a control in antiviral screening tests with coxsackievirus B3 (CVB3)in RNA isolation to dissociate nucleoproteins and inhibit RNase

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri free-flowing product number RDD001.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Packaging

25, 100, 500 g in poly bottle

G4505-1KT:Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

2, 10, 25 kg in poly drum

1 kg in poly bottle

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

absorption≤0.10 at 260 nm in H2O at 6 M
assay≥99% (titration)
biological sourcesynthetic (organic)
colorwhite
density1.3 g/cm3 (lit.)
formcrystalline powder
Gene Informationhuman ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)
impurities≤0.3% water (Karl Fischer)
InChI keyPJJJBBJSCAKJQF-UHFFFAOYSA-N
InChI1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
mp180-185 °C (lit.)
pH(25 °C, 4.6 - 6 at 573 g/L)
Quality Level400
SMILES stringCl[H].NC(N)=N
solubilityH2O: 6 M
technique(s)RNA extraction: suitable
Code
Description
Unit Size
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Qty
G4505-100G
Unit:100G
List Price: $88.45
Source:List Price
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G4505-25G
Unit:25G
List Price: $44.66
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G4505-10KG
Unit:10KG
List Price: $4,089.00
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G4505-1KG
Unit:1KG
List Price: $459.65
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G4505-25KG
Unit:25KG
List Price: $9,744.00
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G4505-2KG
Unit:2KG
List Price: $885.95
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Cas Number50-01-1
This product has met the following criteria: