Brain Heart Infusion Agar, for microbiology

Code: 70138-500G D2-231

Not available outside of the UK & Ireland.

Application

BHI agar is a general-purpose medium used for primary isolation of pathogenic aerobic bacteria from clinical specimens and for the cultivation and maintenance of ...


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$97.59 EACH

Not available outside of the UK & Ireland.

Application

BHI agar is a general-purpose medium used for primary isolation of pathogenic aerobic bacteria from clinical specimens and for the cultivation and maintenance of fastidious microorganisms from food, animal feed, water, and other samplesDefibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Rosenow devised the original medium by adding brain tissue to dextrose agar. These media are nutritious and well buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. Addition of ascites permits the cultivation of Gonococci. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. This medium is to be used for the selective isolation of fastidious fungi (especially of Histoplasma capsulatum and Blastomyces), the growth of the accompanying bacterial and saprophytic yeasts and molds can be almost completely suppressed by adding the following recommended components mixtures: 12 mg Penicillin and 40 ug Streptomycin per liter; 50 mg Chloramphenicol and 500mg Cycloheximide per liter; 50 mg Gentamicin and/or 50 mg Chloramphenicol per liter.This medium is less suited for identifying haemolytic forms when blood is added due to its glucose content. While handling Histoplasma capsulatum extreme care should be taken to avoid dissemination of its infective spores. The culture should be examined in a closed filtered air cabinet.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Selection for vancomycin resistance in clinical isolates of Staph. haemolyticus

Preparation Note

Suspend 52 g in 1 litre of distilled water. Boil to dissolve the medium completely. Distribute into tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes.

application(s)microbiology, clinical testingenvironmentalfood and beverageslife science and biopharmawater monitoring
compositionheart extract, 9.7 g/L, dextrose, 2.0 g/L, sodium chloride, 5.0 g/L, agar, 15 g/L, brain extract, 7.8 g/L, proteose peptone, 10.0 g/L, disodium phosphate, 2.5 g/L
final pH7.4±0.2 (25 °C)
formpowder
manufacturer/tradenameNutriSelect® Plus
pH7.2-7.6 (25 °C)
product lineBioChemika
Quality Level200
shelf lifelimited shelf life, expiry date on the label
sterilitynon-sterile
storage temp.10-30°C
suitabilityHaemophilus spp., nonselective for Escherichia coli, nonselective for Neisseria spp., Blastomyces spp., nonselective for Candida spp., nonselective for bacteria (General Media), Ajellomyces spp., nonselective for Streptococcus spp., Erysipelothrix spp., nonselective for Staphylococcus spp., nonselective for Lactobacillus spp., Pneumococcus spp., Enterococcus spp., nonselective for Clostridium spp., nonselective for Shigella spp., nonselective for coliforms
technique(s)microbiological culture: suitable, microbe id | specific enzyme detection: suitable
This product has met the following criteria: