Not available outside of the UK & Ireland.

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

3,3′,5,5′-Tetramethylbenzidine has been used:as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stainsthe lactobacillicolonies blue in the presence of O2as a substrate solution for ELISA (enzyme-linked immunosorbent assay)in myeloperoxidase activity assay to quantify neutrophil

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in insitu free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Packaging

1, 5, 100 g in glass bottle

Bottomless glass bottle. Contents are inside inserted fused cone.

100 mg in glass bottle