Amersham ECL Western Blotting System

Code: rpn2108 D2-84

Amersham ECL Western blotting detection kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse ...


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£303.00 EACH
£363.60 inc. VAT

Amersham ECL Western blotting detection kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL detection reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera. ECL Western blotting system is the world’s most widely used and referenced chemiluminescent immunodetection system.

  • Convenient kit that includes ECL detection reagent, HRP-conjugated antibodies, and blocking agent.
  • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
  • Optimized system provides rapid, sensitive results using simple protocols.
  • Designed for use with nitrocellulose and PVDF membranes.
  • High sensitivity: Up to 10 times more sensitive than colorimetric methods.
  • Rapid: results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
  • Easy reprobing: save time and materials by reprobing on membranes up to 10 times.
  • Quantifiable: linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
  • Non-radioactive-no specialised facilities needed
  • Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

PackFor detecting 1000cm2
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