Sucrose Assay Kit, sufficient for 20 assays

Code: sca20-1kt D2-231

Not available outside of the UK & Ireland.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Gluc...


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Your Price
$205.90 1KIT
$247.08 inc. VAT

Not available outside of the UK & Ireland.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

analyte chemical class(es)sugars (sucrose)
application(s)food and beveragesgeneral analytical
Quality Level100
storage temp.2-8°C
technique(s)photometry: suitable
usagesufficient for 20 assays
This product has met the following criteria: