Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range Mr~ 5K to ~ 5M.

• Wide fractionation range: optimised to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments
• Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity
• High flow rates: rigid beads give excellent pressure/flow properties
• Enhanced performance: improved resolution and runtime compared to predecessor

Superose 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose 6 Increase columns deliver higher resolution protein purification in shorter runtimes.

Suitable for both preparative and analytical applications, Superose 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Superose 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding Mr ~400 000. The optimal fractionation range of Superdex 200 Increase is complementary, providing higher resolution for proteins with molecular weights below Mr ~400 000.

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.