Not available outside of the UK & Ireland.

Features and Benefits

Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression Highly reproducible, consistent product performance Provides cleaner samples for longer column life and less instrument downtime Available in 96-well and 1 mL cartridge dimensions

General description

HybridSPE-PLus is the next generation of HybridSPE-Phospholipid technology. The first of its kind, HybridSPE-Phospholipid technology was first introduced in 2008. It fuses the simple, standardized methodology of traditional protein precipitation with the specificity of solid phase extraction (SPE) for the simultaneous removal of proteins and phospholipids from biological samples prior to LC-MS analysis. Unlike other phospholipid removal products that use a hydrophobic retention mechanism to remove phsopholipids from biological samples, HybridSPE-Phospholipid technology uses a unique Lewis acid/base retention mechanism that allows it to separate phospholipids from even very hydrophobic analytes, such as vitamins, which are often retained along with phospholipids on competitive products.Our HybridSPE-PLus products offer the same time-proven chemistry of our traditional HybridSPE-Phospholipid products coupled with manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have allowed us to increase flow consistency and reduce sample hold up volumes, further improving analyte recoveries and assay reproducibility. We invite customers to take advantage of the newest technology; HybridSPE-PLus.

Legal Information

HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC