Dextrin Sepharose High Performance 25 ml

Code: 28935597 D2-84

Dextrin Sepharose High Performance is a robust and stable affinity resin for purification of proteins tagged with maltose binding protein (MBP).

  • Fast, one-step purification of MBP...


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    £1,002.00 EACH
    £1,202.40 inc. VAT

Dextrin Sepharose High Performance is a robust and stable affinity resin for purification of proteins tagged with maltose binding protein (MBP).

  • Fast, one-step purification of MBP-tagged proteins.
  • High-resolution purification and elution in narrow peaks, minimizing the need for further concentration steps.
  • Can be used for repeated purification: can be easily regenerated using 0.5M NaOH.
  • Physiological conditions and mild elution preserve target protein activity.

Purifying proteins tagged with maltose binding protein

Recombinant proteins are engineered with an MBP-tag to facilitate detection, isolation, and purification procedures. In addition, this affinity tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein.

Purification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.

Dextrin Sepharose High Performance MBP resin

The small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5M NaOH, allowing the same column to be used to purify different proteins.

Dextrin Sepharose High Performance
Flow velocity, cm/h<<300
LigandDextrin
Particle size, d50V~34µm
MatrixHighly cross-linked agarose, 6%
Binding Capacity/mL Chromatography MediumApprox. 7mg MBP- ΔSal/mL resin Approx. 16mg MBP- βGal/mL resin
pH stability, CIP2 - 13
Storage2 to 8°C, 20% Ethanol
Chemical stabilityStable in commonly used aqueous buffers, 0.5M NaOH (regeneration and cleaning)
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