GSTrap FF 2 x 1mL

Code: 17513002 D2-84

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

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    £179.00 EACH
    £214.80 inc. VAT

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

  • Fast and simple one-step purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose 4 Fast Flow provides good flow properties.

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.

GSTrap FF 5 x 1mLGSTrap FF 2 x 1mLGSTrap FF 1 x 5mLGSTrap FF 5 x 5mL
ApplicationFor the purification of GST-tagged proteins from bacterial lysates and other glutathioine S-transferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
Bed Dims, mm7 x 257 x 2516 x 2516 x 25
Bed Height, mm25252525
Bed Volume1155
Column i.d.771616
Flow Rate, mL/min<4<4<20<20
Storage4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70spi)5 bar [0.5MPa] (70spi)5 bar [0.5MPa] (70spi)5 bar [0.5MPa] (70spi)
Chromatography TechniqueGST-tagged protein purificationGST-tagged protein purificationGST-tagged protein purificationGST-tagged protein purification
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17513001
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17513101
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17513102
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