Benzamidine Sepharose 4 Fast Flow (High Sub) 25 ml

Code: 17512310 D2-84

Benzamidine Sepharose 4 Fast Flow (High Sub) is an excellent tool for removal of proteolytic activity from a protein

  • Benzamidine Sepharose 4 Fast Flow (high sub) is based on highl...


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    £472.00 EACH
    £566.40 inc. VAT

Benzamidine Sepharose 4 Fast Flow (High Sub) is an excellent tool for removal of proteolytic activity from a protein

  • Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity
  • p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.

Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates

Benzamidine Sepharose 4 Fast Flow (High Sub)
Flow velocity, cm/h30 - 300
Ligandp-aminobenzamidine [pABA]
Particle size, d50V~90µm
MatrixHighly cross-linked 4% agarose
Ligand Coupling MethodAmide
Ligand density>12µmol p-aminobenzamidine/mL medium
pH stability, operational2 - 8
pH stability, CIP1 - 9
Pressure/Flow Specificationbase matrix min 150cm/h, 100kPa, XK 50/60 column, bed height 25cm
Storage2 to 8°C, 0.05M Sodium Acetate, pH 4.0 in 20% Ethanol
Chemical stabilityCommonly used aqueous buffers
Chromatography techniqueGroup specific affinity
This product has met the following criteria: