IMAC Sepharose 6 Fast Flow 100 ml

Code: 17092108 D2-84

IMAC Sepharose 6 Fast Flow is an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions in batch mode or when scale up is needed.

  • Supplied uncharged...


    read more

    Your Price
    £1,110.00 EACH
    £1,332.00 inc. VAT

IMAC Sepharose 6 Fast Flow is an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions in batch mode or when scale up is needed.

  • Supplied uncharged, for customized metal ion charging and optimized selectivity.
  • Suitable for purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
  • Based on well-established Sepharose Fast Flow resins.
  • High chemical stability enables proven CIP and sanitisation protocols.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.

Uncharged IMAC resin for his-tagged and untagged proteins

IMAC Sepharose 6 Fast Flow is a member of Cytiva's range of immobilized metal ion affinity chromatography (IMAC) resins, designed for high-quality capture and intermediate native or recombinant protein purification. This resin is commonly used to purify his-tagged proteins, but also untagged recombinant or native proteins.

Resin is composed of cross-linked 6% agarose beads modified with a chelating ligand, which is immobilized to the base matrix. The highly crosslinked resin enables high flow rates and productivity. Resin is supplied uncharged, allowing the user to charge with the metal ion that has optimized selectivity for their target protein.

IMAC Sepharose 6 Fast Flow
Particle Size, um45 - 165
BioProcess resinYes
Particle size, d50V~90µm
Matrix6% cross-linked agarose
Binding Capacity/ml Chromatography Medium~40mg histidine-tagged protein/mL resin when changed with Ni2+
Metal Ion Capacity~25µmol Cu2+/mL medium, ~15µmol Ni2+ or Zn2+/mL medium
pH stability, operational3 - 12
pH stability, CIP2 - 14
Pressure/Flow SpecificationBase matrix: 250-400cm/h, 100kPa, XK 50/60 column, bed height 25cm
Storage4 to 30°C, 20% Ethanol
Chemical stabilityStable in commonly used aqueous buffers - 0.01M HCl, 1M NaOH, 70% acetic acid, 2% SDS. 30% 2-propanol
Code
Description
Unit Size
List Price
Qty
17092007
Unit:EACH
List Price: £1,466.00
Source:List Price
ADD
17092107
Unit:EACH
List Price: £334.00
Source:List Price
ADD
This product has met the following criteria: