Urea, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Code: 15604-50kg D2-231

Not available outside of the UK & Ireland.

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already ...


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Not available outside of the UK & Ireland.

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Urea has been used in the following studies:Synthesis of SnO2 nanoparticles. Synthesis of urethanes, ureides and semicarbazides.Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strainProtein electrophoretic studies.

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.Urea can be prepared from ammonia and carbon-dioxide, via Haber-Bosch process. It can also be used to prepare urea-formaldehyde resins, via condensation reaction.

anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤10 mg/kg
assay99.0-101.0% (calc. on dry substance), 99.0-100.5%
cation tracesFe: ≤0.5 mg/kg
density1.335 g/mL at 25 °C (lit.)
formsolid
gradepuriss.
ign. residue≤0.1% (as SO4)
impurities≤0.1% biuret(carbamylurea), ≤0.001% heavy metals (as Pb), ≤0.04% insoluble in ethanol, alkalic reac. substances, complies, ≤0.05% ammonium (NH4)
InChI keyXSQUKJJJFZCRTK-UHFFFAOYSA-N
InChI1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
loss≤1.0% loss on drying, 105 °C, 1 h
mp132-135 °C, 132-135 °C (lit.)
Quality Level200
qualitymeets analytical specification of Ph. Eur., BP, USP
SMILES stringNC(N)=O
solubilityH2O: soluble 480 g/L at 20 °C
suitabilityin accordance for appearance of solution, in accordance for identity
Cas Number57-13-6
This product has met the following criteria: