Not available outside of the UK & Ireland.

Application

Tumor Necrosis Factor-α (hTNF-α) Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines. Has activating and growth stimulating activities on a variety of normal cells. Has antiviral activity on many cell types in vitro. Can cause tumor necrosis of certain tumors in vivo.

Biochem/physiol Actions

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

General description

Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.

Other Notes

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Physical form

Solution, filtered through 0.2 µm pore size membrane.

Preparation Note

Working concentration: 1 ng/mlFor complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.Working solution: Dilute the concentrated hTNF-α solution (10 µg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.Storage conditions (working solution): -15 to -25 °CIt is recommended to store the solution in aliquots at -15 to -25 °C.Note: Avoid repeated freezing and thawing.

Quality

Purity: >95% (SDS-PAGE)Endotoxin level: ﹤0.1 EU/µg (LAL-test), ﹤10 EU/ml (LAL-test)

Sequence

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Specificity

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Unit Definition

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).