His Gravitrap

Code: 11003399 D2-84

His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilised metal affinity chromatography (IMAC). The ready to use column is prepacked with Ni Sepha...


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£318.00 EACH
£381.60 inc. VAT

His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilised metal affinity chromatography (IMAC). The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives. His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10mL.

  • For fast and simple purification of histidine-tagged proteins
  • Convenient, prepacked gravity-flow column
  • Allows direct purification of both clarified and unclarified cell lysates
  • High binding capacity; approximately 40 mg of pure protein per column
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives

His GraviTrap
Bed volume, mL1
Binding Capacity/ColumnApprox. 40mg histidine-tagged protein/column
Storage4 to 30°C, 20% Ethanol
Chromatography techniqueHistidine-tagged protein purification
Pack size10 x 1mL
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