ROCHE Glutamate-Pyruvate Transaminase (GPT)from pig heart

Code: 10737127001 D2-231

Not available outside of the UK & Ireland.

Application

Synthesis of unnatural L-amino acids from α-keto acids.

General description

Glutamate-pyruvate transaminase is a blood enzyme. I...


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$381.35 EACH

Not available outside of the UK & Ireland.

Application

Synthesis of unnatural L-amino acids from α-keto acids.

General description

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: PyridoxalphosphateStorage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Quality

Contaminants: ﹤ 0.01% GIDH, LDH, and MDH each, ﹤ 0.03% GOT

Unit Definition

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]

biological sourcepig heart
formsuspension
manufacturer/tradenameRoche
optimum pH~8.0
packagingpkg of 2.5 mL (10737127001 [25 mg]), pkg of 1 mL (10105589001 [10 mg])
Quality Level100
shipped inwet ice
specific activity≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
storage temp.2-8°C
This product has met the following criteria: